https://openchemworks.com/Amino acid analysis: methods, application notes and insights from OpenChemWorks, Davis, California.en-ushttps://openchemworks.com/methods/aqc-derivatizationhttps://openchemworks.com/methods/aqc-derivatizationWed, 23 Sep 2026 12:00:00 +0000How AQC tags primary and secondary amino acids for HPLC-UV at 260 nm: the urea chemistry, by-products, borate pH, the heating step and practical limits.https://openchemworks.com/methods/sample-preparationhttps://openchemworks.com/methods/sample-preparationWed, 23 Sep 2026 12:00:00 +0000Why protein comes out before free amino acid analysis: ultrafiltration vs acid or solvent precipitation, dilution into range, and internal standards.https://openchemworks.com/methods/free-vs-total-amino-acidshttps://openchemworks.com/methods/free-vs-total-amino-acidsWed, 23 Sep 2026 12:00:00 +0000Free amino acids are unbound; total ones are measured after acid hydrolysis. What hydrolysis changes, and which your media, label or hydrolysate needs.https://openchemworks.com/methods/reading-an-amino-acid-profilehttps://openchemworks.com/methods/reading-an-amino-acid-profileWed, 23 Sep 2026 12:00:00 +0000How to interpret amino acid analysis results: µM vs mg/L, mol% and ratios, below-LOQ flags, dilution correction, the chromatogram and time courses.https://openchemworks.com/methods/quality-controlhttps://openchemworks.com/methods/quality-controlWed, 23 Sep 2026 12:00:00 +0000The QC behind our amino acid results: internal standard, QC standard each run, detector checks, reagent blanks, spike recovery and below-LOQ flags.https://openchemworks.com/methods/analyte-referencehttps://openchemworks.com/methods/analyte-referenceWed, 23 Sep 2026 12:00:00 +0000Glutamine stability in solution, asparagine deamidation, tryptophan and cysteine chemistry, ammonia sources and the extended panel, analyte by analyte.https://openchemworks.com/notes/cultivated-meat-media-amino-acid-costshttps://openchemworks.com/notes/cultivated-meat-media-amino-acid-costsWed, 23 Sep 2026 12:00:00 +0000What published techno-economic analyses say about media cost in cultivated meat, where amino acids fit, and how spent-media data support cheaper media.https://openchemworks.com/notes/free-amino-acids-in-hydrolysateshttps://openchemworks.com/notes/free-amino-acids-in-hydrolysatesWed, 23 Sep 2026 12:00:00 +0000What a free amino acid profile shows about protein hydrolysates, peptones and yeast extracts that total composition misses: a review of published data.https://openchemworks.com/notes/glutamine-glutamate-ammonia-fed-batchhttps://openchemworks.com/notes/glutamine-glutamate-ammonia-fed-batchWed, 23 Sep 2026 12:00:00 +0000What published CHO studies show about glutamine uptake, glutamine breakdown and ammonia in fed-batch culture, and how to sample and read all three.https://openchemworks.com/insights/amino-acid-analysis-costhttps://openchemworks.com/insights/amino-acid-analysis-costWed, 23 Sep 2026 12:00:00 +0000What drives the price of amino acid analysis (hydrolysis, panel size, preparation, calibration, minimums), our posted prices, a worked estimate and a checklist for the report.https://openchemworks.com/insights/choosing-an-amino-acid-methodhttps://openchemworks.com/insights/choosing-an-amino-acid-methodWed, 23 Sep 2026 12:00:00 +0000Ion-exchange/ninhydrin, AQC, OPA/FMOC, PITC and LC-MS/MS compared on proline coverage, derivative stability, sensitivity, run time and matrix tolerance.