Stop optimizing your culture blind.
Ammonia is climbing on batch 14. Titers are stalling. And nobody can tell you which nutrients depleted — because nobody measured them.
Feed decisions made on guesswork.
You adjust the feed because the last batch worked — or because a paper suggested an additive, or because the vendor’s application note said so. The culture tells you the outcome weeks later. It never tells you what it needed.
The instrument vendors will happily sell you the tools to measure amino acids yourself. A column here, a kit there, a method to develop on your own time. What they don’t sell is the answer.
So the most expensive variable in your process — the feed — gets optimized by trial and error.
The missing measurement: what’s left in the media.
A spent-media free amino acid and ammonia panel shows which amino acids your culture actually consumed and how much ammonia built up — per batch, per timepoint. Feed decisions stop being guesses. See what an amino acid panel tells you for how to read the numbers.
Related
More on this job, and the pages around it.
Ammonia in Cell Culture
Is ammonia killing your culture? What rising ammonia means and how to track it.
Explore →Job pageAmino Acid Spiking Test
Is your protein spiked? Every amino acid measured individually — the measurement Kjeldahl can’t make.
Explore →ApplicationCell culture media
Glutamine, ammonia, asparagine and essential amino acids in fresh, fed and spent media, for feed design and lot comparison.
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